Malanchuk, OM;
Bdzhola, AV;
Tykhonkova, IO;
Gout, IT;
Filonenko, VV;
(2022)
Monoclonal antibodies to Coenzyme A.
Biopolymers & Cell
, 38
(4)
pp. 215-223.
10.7124/bc.000A7F.
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Abstract
Aim. To generate monoclonal antibodies to Coenzyme A (CoA) with antigenic epitope specificity different to previously developed anti-CoA mAb (1F10) by introducing alternative approach for antigen preparation animal immunization. Methods. Cross-linking of CoA to Keyhole Limpet Hemocyanin (KLH) and to Bovine serum albumin (BSA) using reversible chemical crosslinker SPDP. Hybridoma technique. Western blot analysis. Immunoprecipitation. ELISA. Results. Monoclonal antibody (A11) that specifically recognizes CoA in various immunoassays and has the same antigenic epitope specificity as existing anti-CoA mAb (1F10) has been generated. Conclusions. Application of different ways for CoA conjugation to the carrier proteins to create an antigen for immunization does not affect the antigenic epitope specificity of generated anti-CoA antibodies most probably due to the existence of only one immunogenic epitope in CoA molecule.
| Type: | Article |
|---|---|
| Title: | Monoclonal antibodies to Coenzyme A. |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.7124/bc.000A7F |
| Publisher version: | http://dx.doi.org/10.7124/bc.000a7f |
| Language: | English |
| Additional information: | © 2022 O. M. Malanchuk et al.; Published by the Institute of Molecular Biology and Genetics, NAS of Ukraine on behalf of Biopolymers and Cell. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited |
| Keywords: | CoA, hybridoma technique, monoclonal antibody, SPDP crosslinking |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10190568 |
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