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Minimum intensity projection of embossed quadrant-detection images for improved photoreceptor mosaic visualisation

Kalitzeos, A; Michaelides, M; Dubra, A; (2024) Minimum intensity projection of embossed quadrant-detection images for improved photoreceptor mosaic visualisation. Frontiers in Ophthalmology , 4 10.3389/fopht.2024.1349297. Green open access

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Abstract

Non-confocal split-detection imaging reveals the cone photoreceptor inner segment mosaic in a plethora of retinal conditions, with the potential of providing insight to ageing, disease, and response to treatment processes, in vivo, and allows the screening of candidates for cell rescue therapies. This imaging modality complements confocal reflectance adaptive optics scanning light ophthalmoscopy, which relies on the waveguiding properties of cones, as well as their orientation toward the pupil. Split-detection contrast, however, is directional, with each cone inner segment appearing as opposite dark and bright semicircles, presenting a challenge for either manual or automated cell identification. Quadrant-detection imaging, an evolution of split detection, could be used to generate images without directional dependence. Here, we demonstrate how the embossed-filtered quadrant-detection images, originally proposed by Migacz et al. for visualising hyalocytes, can also be used to generate photoreceptor mosaic images with better and non-directional contrast for improved visualisation. As a surrogate of visualisation improvement between legacy split-detection images and the images resulting from the method described herein, we provide preliminary results of simple image processing routines that may enable the automated identification of generic image features, as opposed to complex algorithms developed specifically for photoreceptor identification, in pathological retinas.

Type: Article
Title: Minimum intensity projection of embossed quadrant-detection images for improved photoreceptor mosaic visualisation
Open access status: An open access version is available from UCL Discovery
DOI: 10.3389/fopht.2024.1349297
Publisher version: http://dx.doi.org/10.3389/fopht.2024.1349297
Language: English
Additional information: Copyright © 2024 Kalitzeos, Michaelides and Dubra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Keywords: adaptive optics, quadrant-detection, non-confocal, photoreceptors, split-detection, minimum intensity projection, contrast
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10190520
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