McCoubrey, LE;
Ferraro, F;
Seegobin, N;
Verin, J;
Alfassam, HA;
Awad, A;
Marzorati, M;
... Basit, AW; + view all
(2024)
Poly(D,L-lactide-co-glycolide) particles are metabolised by the gut microbiome and elevate short chain fatty acids.
Journal of Controlled Release
, 369
pp. 163-178.
10.1016/j.jconrel.2024.03.039.
Preview |
Text
1-s2.0-S0168365924001925-main.pdf - Published Version Download (9MB) | Preview |
Abstract
The production of short chain fatty acids (SCFAs) by the colonic microbiome has numerous benefits for human health, including maintenance of epithelial barrier function, suppression of colitis, and protection against carcinogenesis. Despite the therapeutic potential, there is currently no optimal approach for elevating the colonic microbiome's synthesis of SCFAs. In this study, poly(D,L-lactide-co-glycolide) (PLGA) was investigated for this application, as it was hypothesised that the colonic microbiota would metabolise PLGA to its lactate monomers, which would promote the resident microbiota's synthesis of SCFAs. Two grades of spray dried PLGA, alongside a lactate bolus control, were screened in an advanced model of the human colon, known as the M-SHIME® system. Whilst the high molecular weight (Mw) grade of PLGA was stable in the presence of the microbiota sourced from three healthy humans, the low Mw PLGA (PLGA 2) was found to be metabolised. This microbial degradation led to sustained release of lactate over 48 h and increased concentrations of the SCFAs propionate and butyrate. Further, microbial synthesis of harmful ammonium was significantly reduced compared to untreated controls. Interestingly, both types of PLGA were found to influence the composition of the luminal and mucosal microbiota in a donor-specific manner. An in vitro model of an inflamed colonic epithelium also showed the polymer to affect the expression of pro- and anti-inflammatory markers, such as interleukins 8 and 10. The findings of this study reveal PLGA's sensitivity to enzymatic metabolism in the gut, which could be harnessed for therapeutic elevation of colonic SCFAs.
| Type: | Article |
|---|---|
| Title: | Poly(D,L-lactide-co-glycolide) particles are metabolised by the gut microbiome and elevate short chain fatty acids |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jconrel.2024.03.039 |
| Publisher version: | https://doi.org/10.1016/j.jconrel.2024.03.039 |
| Language: | English |
| Additional information: | Copyright © 2024 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Colonic drug delivery, Metabolomics, Microbiome medicine, Microbiome sequencing, Nanoparticles and microparticles, PLGA, Spray drying |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmaceutics |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10190250 |
Archive Staff Only
 |
View Item |
