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Critical importance of DNA binding for CSL protein functions in fission yeast

Marešová, Anna; Oravcová, Martina; Rodríguez-López, María; Hradilová, Miluše; Zemlianski, Viacheslav; Häsler, Robert; Hernández, Pablo; ... Převorovský, Martin; + view all (2024) Critical importance of DNA binding for CSL protein functions in fission yeast. Journal of Cell Science , Article jcs.261568. 10.1242/jcs.261568. Green open access

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Abstract

CSL (CBF1/RBP-Jκ/Suppressor of Hairless/LAG-1) proteins are conserved transcription factors found in animals and fungi. In fission yeast, they regulate various cellular processes, including cell cycle progression, lipid metabolism, and cell adhesion. CSL proteins bind to DNA through their N-terminal Rel-like domain and central beta-trefoil domain. Here, we investigated the importance of DNA binding for CSL functions in the fission yeast Schizosaccharomyces pombe. We created CSL mutants with disrupted DNA binding and found that the vast majority of CSL functions depend on intact DNA binding. Specifically, DNA binding is crucial for the regulation of cell adhesion, lipid metabolism, cell cycle progression, long non-coding RNA expression, and genome integrity maintenance. Interestingly, perturbed lipid metabolism leads to chromatin structure changes, potentially linking lipid metabolism to the diverse CSL-associated phenotypes. Our study highlights the critical role of DNA binding for CSL protein functions in fission yeast.

Type: Article
Title: Critical importance of DNA binding for CSL protein functions in fission yeast
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/jcs.261568
Publisher version: https://doi.org/10.1242/jcs.261568
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: CSL proteins, Cell adhesion, Chromatin structure, DNA binding, Fission yeast, Genome integrity, Lipid metabolism
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10189464
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