Jha, D;
Blennow, K;
Zetterberg, H;
Savas, JN;
Hanrieder, J;
(2024)
Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies.
Journal of Mass Spectrometry
, 59
(3)
, Article e5008. 10.1002/jms.5008.
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Abstract
Given the complexity of nervous tissues, understanding neurochemical pathophysiology puts high demands on bioanalytical techniques with respect to specificity and sensitivity. Mass spectrometry imaging (MSI) has evolved to become an important, biochemical imaging technology for spatial biology in biological and translational research. The technique facilitates comprehensive, sensitive elucidation of the spatial distribution patterns of drugs, lipids, peptides, and small proteins in situ. Matrix-assisted laser desorption ionization (MALDI)-based MSI is the dominating modality due to its broad applicability and fair compromise of selectivity, sensitivity price, throughput, and ease of use. This is particularly relevant for the analysis of spatial lipid patterns, where no other comparable spatial profiling tools are available. Understanding spatial lipid biology in nervous tissue is therefore a key and emerging application area of MSI research. The aim of this review is to give a concise guide through the MSI workflow for lipid imaging in central nervous system (CNS) tissues and essential parameters to consider while developing and optimizing MSI assays. Further, this review provides a broad overview of key developments and applications of MALDI MSI-based spatial neurolipidomics to map lipid dynamics in neuronal structures, ultimately contributing to a better understanding of neurodegenerative disease pathology.
| Type: | Article |
|---|---|
| Title: | Spatial neurolipidomics—MALDI mass spectrometry imaging of lipids in brain pathologies |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/jms.5008 |
| Publisher version: | https://doi.org/10.1002/jms.5008 |
| Language: | English |
| Additional information: | © 2024 The Authors. Journal of Mass Spectrometry published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | Alzheimer's disease (AD), amyloid, gangliosides, inositols, lysolipids, mass spectrometry imaging (MSI), matrix-assisted laser desorption ionization (MALDI), neurolipidomics, Niemann–Pick disease type C1 (NPC1), Parkinson's disease (PD), spatial biology, sphingolipids |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10189063 |
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