Swire, Matthew;
Ffrench-Constant, Charles;
(2020)
Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter.
bio-protocol
, 10
(20)
, Article e3792. 10.21769/BioProtoc.3792.
Preview |
PDF
Bio-protocol3792.pdf - Published Version Download (2MB) | Preview |
Abstract
Oligodendrocytes generate distinct patterns of myelination throughout the CNS. Variations in myelination along axons may enable neurons to fine-tune conduction velocities and alter signal synchronisation. Here we outline a staining protocol permitting the assessment of the number and length of myelin sheaths formed by oligodendrocyte in the mouse grey matter. This protocol enables the investigation of myelination without the need for reporter mice or technically challenging protocols, aiding the investigation of factors influencing myelin production in the brain.
| Type: | Article |
|---|---|
| Title: | Staining and Quantitative Analysis of Myelinating Oligodendrocytes in the Mouse Grey Matter |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.21769/BioProtoc.3792 |
| Publisher version: | http://dx.doi.org/10.21769/bioprotoc.3792 |
| Language: | English |
| Additional information: | © The Authors 2023. Swire and ffrench-Constant. This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Oligodendrocyte, Myelin, Myelination, CNS, Immunofluorescence |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Wolfson Inst for Biomedical Research |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10187828 |
Loading...
Loading...Loading...Loading...Archive Staff Only
 |
View Item |
