UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system for in vivo mouse retina imaging

Zhang, Pengfei; Wahl, Daniel J; Mocci, Jacopo; Miller, Eric B; Bonora, Stefano; Sarunic, Marinko; Zawadzki, Robert J; (2023) Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system for in vivo mouse retina imaging. Biomedical Optics Express , 14 (1) pp. 299-314. 10.1364/BOE.473447. Green open access

[thumbnail of boe-14-1-299.pdf]
Preview
Text
boe-14-1-299.pdf - Published Version

Download (11MB) | Preview

Abstract

Optical coherence tomography (OCT) and scanning laser ophthalmoscopy (SLO) are imaging technologies invented in the 1980s that have revolutionized the field of in vivo retinal diagnostics and are now commonly used in ophthalmology clinics as well as in vision science research. Adaptive optics (AO) technology enables high-fidelity correction of ocular aberrations, resulting in improved resolution and sensitivity for both SLO and OCT systems. The potential of gathering multi-modal cellular-resolution information in a single instrument is of great interest to the ophthalmic imaging community. Although similar instruments have been developed for imaging the human retina, developing such a system for mice will benefit basic science research and should help with further dissemination of AO technology. Here, we present our work integrating OCT into an existing mouse retinal AO-SLO system, resulting in a multi-modal AO-enhanced imaging system of the living mouse eye. The new system allows either independent or simultaneous data acquisition of AO-SLO and AO-OCT, depending on the requirements of specific scientific experiments. The system allows a data acquisition speed of 200 kHz A-scans/pixel rate for OCT and SLO, respectively. It offers ∼6 µm axial resolution for AO-OCT and a ∼1 µm lateral resolution for AO-SLO-OCT imaging.

Type: Article
Title: Adaptive optics scanning laser ophthalmoscopy and optical coherence tomography (AO-SLO-OCT) system for in vivo mouse retina imaging
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1364/BOE.473447
Publisher version: http://dx.doi.org/10.1364/boe.473447
Language: English
Additional information: © 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement
Keywords: Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemical Research Methods, Optics, Radiology, Nuclear Medicine & Medical Imaging, Biochemistry & Molecular Biology, ULTRAHIGH-RESOLUTION, LIGHT, REFLECTANCE, DISPERSION, NEURONS, CELLS, SPEED
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10186901
Downloads since deposit
4Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item