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Condensin controls cellular RNA levels through the accurate segregation of chromosomes instead of directly regulating transcription

Hocquet, Clemence; Robellet, Xavier; Modolo, Laurent; Sun, Xi-Ming; Burny, Claire; Cuylen-Haering, Sara; Toselli, Esther; ... Bernard, Pascal; + view all (2018) Condensin controls cellular RNA levels through the accurate segregation of chromosomes instead of directly regulating transcription. eLIFE , 7 , Article e38517. 10.7554/eLife.38517. Green open access

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Abstract

Condensins are genome organisers that shape chromosomes and promote their accurate transmission. Several studies have also implicated condensins in gene expression, although any mechanisms have remained enigmatic. Here, we report on the role of condensin in gene expression in fission and budding yeasts. In contrast to previous studies, we provide compelling evidence that condensin plays no direct role in the maintenance of the transcriptome, neither during interphase nor during mitosis. We further show that the changes in gene expression in post-mitotic fission yeast cells that result from condensin inactivation are largely a consequence of chromosome missegregation during anaphase, which notably depletes the RNA-exosome from daughter cells. Crucially, preventing karyotype abnormalities in daughter cells restores a normal transcriptome despite condensin inactivation. Thus, chromosome instability, rather than a direct role of condensin in the transcription process, changes gene expression. This knowledge challenges the concept of gene regulation by canonical condensin complexes.

Type: Article
Title: Condensin controls cellular RNA levels through the accurate segregation of chromosomes instead of directly regulating transcription
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.38517
Publisher version: http://dx.doi.org/10.7554/elife.38517
Language: English
Additional information: Copyright Hocquet et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
Keywords: Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, FISSION YEAST, SELECTIVE ELIMINATION, GENE-EXPRESSION, READ ALIGNMENT, MESSENGER-RNAS, COMPLEXES, COHESIN, CHROMATIN, EXOSOME, DNA
URI: https://discovery.ucl.ac.uk/id/eprint/10185984
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