King, Claire;
Elsherif, Nusaybah;
Kirwan, Ruaidhri;
Schilling, Clare;
Hall, Gillian;
Morgan, Peter;
Collins, Lisette;
... Thavaraj, Selvam; + view all
(2021)
Serial step sections at narrow intervals with immunohistochemistry are required for accurate histological assessment of sentinel lymph node biopsy in oral squamous cell carcinoma.
Head and Neck
, 43
(10)
pp. 2985-2993.
10.1002/hed.26784.
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Abstract
Background: Sentinel lymph node (SLN) biopsy is an accurate staging modality in early oral squamous cell carcinoma (OSCC), but its accuracy relies on labor-intensive histopathology protocols. We sought to determine whether serial step sections with immunohistochemistry (SSSIHC) at narrow intervals of the entire SLN are required to accurately exclude metastasis. Methods: Consecutive SLN biopsies over a 13-year period were retrospectively evaluated. If the index section was negative for carcinoma, the entire SLN was subjected to SSSIHC at 150 μm intervals. The first section level and total number of section levels to contain carcinoma were recorded. Results: One hundred and eighteen SLN+ from 90 patients were included. SSSIHC upstaged the nodal status in 19.5% of patients. Metastasis was identified in 16.7% and 10.2% beyond section levels 4 and 6, respectively. Among SLNs requiring SSSIHC, 47.5% contained carcinoma in a single section level. Conclusion: SSSIHC of the entire SLN at 150 μm intervals are required to identify occult metastasis in OSCC.
| Type: | Article |
|---|---|
| Title: | Serial step sections at narrow intervals with immunohistochemistry are required for accurate histological assessment of sentinel lymph node biopsy in oral squamous cell carcinoma |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1002/hed.26784 |
| Publisher version: | http://dx.doi.org/10.1002/hed.26784 |
| Language: | English |
| Additional information: | © 2021 The Authors. Head & Neck published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| Keywords: | isolated tumor cells, micrometastasis, oral squamous cell carcinoma, sentinel node, serial step sections |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10184636 |
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