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In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae

Galindo-Rodriguez, Giuseppe R; Santoyo-Garcia, Jorge H; Rios-Solis, Leonardo; Dimartino, Simone; (2023) In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae. Preparative Biochemistry & Biotechnology 10.1080/10826068.2023.2207204. (In press). Green open access

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Abstract

In this study, an engineered strain of Saccharomyces cerevisiae was used to produce taxadiene, a precursor in the biosynthetic pathway of the anticancer drug paclitaxel. Taxadiene was recovered in situ with the polymeric adsorbent Diaion © HP-20. Here we tested two bioreactor configurations and adsorbent concentrations to maximize the production and recovery of taxadiene. An external recovery configuration (ERC) was performed with the integration of an expanded bed adsorption column, whereas the internal recovery configuration (IRC) consisted in dispersed beads inside the bioreactor vessel. Taxadiene titers recovered in IRC were higher to ERC by 3.4 and 3.5 fold by using 3% and 12% (w/v) adsorbent concentration respectively. On the other hand, cell growth kinetics were faster in ERC which represents an advantage in productivity (mg of taxadiene/L*h). High resin bead concentration (12% w/v) improved the partition of taxadiene onto the beads up to 98%. This result represents an advantage over previous studies using a 3% resin concentration where the partition of taxadiene on the beads was around 50%. This work highlights the potential of in situ product recovery to improve product partition, reduce processing steps and promote cell growth. Nevertheless, a careful design of bioreactor configuration and process conditions is critical.

Type: Article
Title: In situ recovery of taxadiene using solid adsorption in cultivations with Saccharomyces cerevisiae
Open access status: An open access version is available from UCL Discovery
DOI: 10.1080/10826068.2023.2207204
Publisher version: https://doi.org/10.1080/10826068.2023.2207204
Language: English
Additional information: Copyright © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4. 0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.
Keywords: Expanded bed adsorption; In situ product recovery; Solid adsorbents; Saccharomyces cerevisiae; Taxadiene
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10183086
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