UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

MRI-guided radiotherapy of the SK-N-SH neuroblastoma xenograft model using a small animal radiation research platform

Corroyer-Dulmont, A; Falzone, N; Kersemans, V; Thompson, J; Hill, M; Danny Allen, P; Beech, J; ... Vallis, KA; + view all (2017) MRI-guided radiotherapy of the SK-N-SH neuroblastoma xenograft model using a small animal radiation research platform. British Journal of Radiology , 90 (1069) , Article 20160427. 10.1259/bjr.20160427. Green open access

[thumbnail of corroyer-dulmont-et-al-2016.pdf]
Preview
Text
corroyer-dulmont-et-al-2016.pdf - Other

Download (1MB) | Preview

Abstract

Objective: Neuroblastoma has one of the lowest survival rates of all childhood cancers, despite the use of intensive treatment regimens. Preclinical models of neuroblastoma are essential for testing new multimodality protocols, including those that involve radiotherapy (RT). The aim of this study was to develop a robust method for RT planning and tumour response monitoring based on combined MRI and cone-beam CT (CBCT) imaging and to apply it to a widely studied mouse xenograft model of neuroblastoma, SK-N-SH. Methods: As part of a tumour growth inhibition study, SK-N-SH xenografts were generated in BALB/c nu/nu mice. Mice (n58) were placed in a printed MR-And CT-compatible plastic cradle, imaged using a 4.7-T MRI scanner and then transferred to a small animal radiation research platform (SARRP) irradiator with on-board CBCT. MRI/CBCT co-registration was performed to enable RT planning using the soft-Tissue contrast afforded by MRI prior to delivery of RT (5Gy). Tumour response was assessed by serial MRI and calliper measurements. Results: SK-N-SH xenografts formed soft, deformable tumours that could not be differentiated from surrounding normal tissues using CBCT. MR images, which allowed clear delineation of tumours, were successfully coregistered with CBCT images, allowing conformal RT to be delivered. MRI measurements of tumour volume 4 days after RT correlated strongly with length of survival time. Conclusion: MRI allowed precision RT of SK-N-SH tumours and provided an accurate means of measuring tumour response. Advances in knowledge: MRI-based RT planning of murine tumours is feasible using an SARRP irradiator.

Type: Article
Title: MRI-guided radiotherapy of the SK-N-SH neuroblastoma xenograft model using a small animal radiation research platform
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1259/bjr.20160427
Publisher version: http://dx.doi.org/10.1259/bjr.20160427
Language: English
Additional information: © 2016 The Authors. Published by the British Institute of Radiology under the terms of the Creative Commons Attribution 4.0 Unported License http://creativecommons. org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Keywords: Analysis of Variance, Animals, Disease Models, Animal, Early Diagnosis, Heterografts, Humans, Image Processing, Computer-Assisted, Magnetic Resonance Imaging, Mice, Mice, Nude, Neuroblastoma, Predictive Value of Tests, Radiotherapy Dosage, Radiotherapy Planning, Computer-Assisted, Risk Assessment, Tumor Burden
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Oncology
URI: https://discovery.ucl.ac.uk/id/eprint/10182931
Downloads since deposit
4Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item