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Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites

Atkinson, Laura; Lee, Jack CD; Lennon, Alexander; Shah, Divya; Storey, Nathaniel; Morfopoulou, Sofia; Harris, Kathryn A; ... Brown, Julianne R; + view all (2023) Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites. Heliyon , 9 (9) , Article E19854. 10.1016/j.heliyon.2023.e19854. Green open access

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Abstract

Metagenomic next-generation sequencing (mNGS) is an untargeted technique capable of detecting all microbial nucleic acid within a sample. This protocol outlines our wet laboratory method for mNGS of cerebrospinal fluid (CSF) specimens and tissues from sterile sites. We use this method routinely in our clinical service, processing 178 specimens over the past 2.5 years in a laboratory that adheres to ISO:15189 standards. We have successfully used this protocol to diagnose multiple cases of encephalitis and hepatitis.

Type: Article
Title: Untargeted metagenomics protocol for the diagnosis of infection from CSF and tissue from sterile sites
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.heliyon.2023.e19854
Publisher version: https://doi.org/10.1016/j.heliyon.2023.e19854
Language: English
Additional information: Copyright © 2023 Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10178152
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