UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors

Marques, MPC; Aranda Hernandez, J; Fernandes, P; (2023) β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors. Methods in Molecular Biology , 2704 pp. 201-219. 10.1007/978-1-0716-3385-4_12. Green open access

[thumbnail of Marques_beta-Sitosterol Bioconversion in Small-Scale Devices_AAM.pdf] Text
Marques_beta-Sitosterol Bioconversion in Small-Scale Devices_AAM.pdf
Access restricted to UCL open access staff until 31 August 2024.

Download (15MB)

Abstract

Small-scale devices are routinely used as low-cost miniaturized bioreactors due to the large number of experiments that can be conducted simultaneously under similar conditions and replicate all functions of bench-scale reactors at dramatically smaller volumes. Microtiter plates, due to the standard footprint, can be integrated with liquid handling systems and associated equipment, expanding considerably their application and use. However, care has to be taken to operate the microtiter plates in optimized mixing and oxygen transfer conditions, preventing medium evaporation in prolonged experiment runs. Recently, to increase data quality, microbioreactors have emerged as an alternative to shaken systems. These systems offer higher degree of control over key process variables and when combined with sensing technology increase dramatically the reliability of translational process data. In this chapter, we describe the production of 4-androstene-3,17-dione (androstenedione (AD)), a key pharmaceutical steroid intermediate, by Mycobacterium sp. NRRL B-3805 via the selective cleavage of the side-chain of β-sitosterol using 24-well microtiter plates and microfluidic microbioreactors.

Type: Article
Title: β-Sitosterol Bioconversion in Small-Scale Devices: From Microtiter Plates to Microfluidic Reactors
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-3385-4_12
Publisher version: https://doi.org/10.1007/978-1-0716-3385-4_12
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.
Keywords: microtiter plate, microbioreactor, Mycobacterium sp. NRRL B-3805, 4-androstene3,17-dione, AD, β-sitosterol, steroid, evaporation, high throughput screening
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10177585
Downloads since deposit
2Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item