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Optimizing Tuberculosis Testing for Basic Laboratories

Ramos, Eric; Schumacher, Samuel G; Siedner, Mark; Herrera, Beatriz; Quino, Willi; Alvarado, Jessica; Montoya, Rosario; ... Evans, Carlton A; + view all (2010) Optimizing Tuberculosis Testing for Basic Laboratories. American Journal of Tropical Medicine and Hygiene (ASTMH Journal) , 83 (4) pp. 896-901. 10.4269/ajtmh.2010.09-0566. Green open access

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Abstract

Optimal tuberculosis testing usually involves sputum centrifugation followed by broth culture. However, centrifuges are biohazardous and scarce in the resource-limited settings where most tuberculosis occurs. To optimize tuberculosis testing for these settings, centrifugation of 111 decontaminated sputum samples was compared with syringe-aspiration through polycarbonate membrane-filters that were then cultured in broth. To reduce the workload of repeated microscopic screening of broth cultures for tuberculosis growth, the colorimetric redox indicator 2,3-diphenyl-5-(2-thienyl) tetrazolium chloride was added to the broth, which enabled naked-eye detection of culture positivity. This combination of filtration and colorimetric growth-detection gave similar results to sputum centrifugation followed by culture microscopy regarding mean colony counts (43 versus 48; P = 0.6), contamination rates (0.9% versus 1.8%; P = 0.3), and sensitivity (94% versus 95%; P = 0.7), suggesting equivalency of the two methods. By obviating centrifugation and repeated microscopic screening of cultures, this approach may constitute a more appropriate technology for rapid and sensitive tuberculosis diagnosis in basic laboratories.

Type: Article
Title: Optimizing Tuberculosis Testing for Basic Laboratories
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.4269/ajtmh.2010.09-0566
Publisher version: https://doi.org/10.4269/ajtmh.2010.09-0566
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
URI: https://discovery.ucl.ac.uk/id/eprint/10176824
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