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Intraperitoneal Injection of Neonatal Mice

Pocratsky, Amanda M; Sleigh, James N; (2023) Intraperitoneal Injection of Neonatal Mice. Bio-protocol , 13 (18) , Article e4826. 10.21769/BioProtoc.4826. Green open access

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Abstract

Administration of substances into neonatal mice is required for early treatment with pre-clinical therapeutics, delivery of recombination-inducing substances and dosing with viruses or toxins, amongst other things. Several injection routes into mouse pups are possible, including intravenous and intracerebroventricular, each with their own advantages and limitations. Here, we describe a simple and rapid protocol for the intraperitoneal injection of neonatal mice for systemic dosing. By detaching a 30 gauge needle from its plastic hub and inserting it into polyethylene tubing attached to a Hamilton syringe, small volumes (1-10 µL) can be accurately injected into the peritoneal cavity of pups aged 1-5 days old. The procedure can be completed within a few minutes, is generally safe and well tolerated by both pups and parents, and can be used in combination with alternative administration routes.

Type: Article
Title: Intraperitoneal Injection of Neonatal Mice
Open access status: An open access version is available from UCL Discovery
DOI: 10.21769/BioProtoc.4826
Publisher version: https://doi.org/10.21769/BioProtoc.4826
Language: English
Additional information: © 2023 The Author(s); This is an open access article under the CC BY-NC license (https://creativecommons.org/licenses/by-nc/4.0/).
Keywords: Adeno-associated virus (AAV), gene therapy, I.P. administration, lentivirus (LV), mouse pup, neonate, systemic delivery, tamoxifen
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
URI: https://discovery.ucl.ac.uk/id/eprint/10175926
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