UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Locational memory of macrovessel vascular cells is transcriptionally imprinted

Spanjersberg, TCF; Oosterhoff, LA; Kruitwagen, HS; van den Dungen, NAM; Vernooij, JCM; Asselbergs, FW; Mokry, M; ... van Steenbeek, FG; + view all (2023) Locational memory of macrovessel vascular cells is transcriptionally imprinted. Scientific reports , 13 (1) , Article 13028. 10.1038/s41598-023-38880-6. Green open access

[thumbnail of s41598-023-38880-6.pdf]
Preview
PDF
s41598-023-38880-6.pdf - Published Version

Download (3MB) | Preview

Abstract

Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems.

Type: Article
Title: Locational memory of macrovessel vascular cells is transcriptionally imprinted
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s41598-023-38880-6
Publisher version: https://doi.org/10.1038/s41598-023-38880-6
Language: English
Additional information: © 2023 Springer Nature Limited. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Animals, Dogs, Endothelial Cells, Aorta, Coronary Vessels, Venae Cavae, Saphenous Vein, Cells, Cultured
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Health Informatics
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Health Informatics > Clinical Epidemiology
URI: https://discovery.ucl.ac.uk/id/eprint/10175728
Downloads since deposit
8Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item