Spanjersberg, TCF;
Oosterhoff, LA;
Kruitwagen, HS;
van den Dungen, NAM;
Vernooij, JCM;
Asselbergs, FW;
Mokry, M;
... van Steenbeek, FG; + view all
(2023)
Locational memory of macrovessel vascular cells is transcriptionally imprinted.
Scientific reports
, 13
(1)
, Article 13028. 10.1038/s41598-023-38880-6.
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Abstract
Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems.
| Type: | Article |
|---|---|
| Title: | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s41598-023-38880-6 |
| Publisher version: | https://doi.org/10.1038/s41598-023-38880-6 |
| Language: | English |
| Additional information: | © 2023 Springer Nature Limited. This article is licensed under a Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Animals, Dogs, Endothelial Cells, Aorta, Coronary Vessels, Venae Cavae, Saphenous Vein, Cells, Cultured |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Health Informatics UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > Institute of Health Informatics > Clinical Epidemiology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10175728 |
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