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The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation

Barlow, Ida L; Mackay, Eirinn; Wheater, Emily; Goel, Aimee; Lim, Sumi; Zimmerman, Steve; Woods, Ian; ... Rihel, Jason; + view all (2023) The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation. eLife , 12 , Article RP87521. 10.7554/elife.87521.3. Green open access

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Abstract

Sleep is a nearly universal feature of animal behaviour, yet many of the molecular, genetic, and neuronal substrates that orchestrate sleep/wake transitions lie undiscovered. Employing a viral insertion sleep screen in larval zebrafish, we identified a novel gene, dreammist (dmist), whose loss results in behavioural hyperactivity and reduced sleep at night. The neuronally expressed dmist gene is conserved across vertebrates and encodes a small single-pass transmembrane protein that is structurally similar to the Na+,K+-ATPase regulator, FXYD1/Phospholemman. Disruption of either fxyd1 or atp1a3a, a Na+,K+-ATPase alpha-3 subunit associated with several heritable movement disorders in humans, led to decreased night-time sleep. Since atpa1a3a and dmist mutants have elevated intracellular Na+ levels and non-additive effects on sleep amount at night, we propose that Dmist-dependent enhancement of Na+ pump function modulates neuronal excitability to maintain normal sleep behaviour.

Type: Article
Title: The zebrafish mutant dreammist implicates sodium homeostasis in sleep regulation
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/elife.87521.3
Publisher version: https://doi.org/10.7554/eLife.87521
Language: English
Additional information: © Copyright Barlow et al. This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10175516
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