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Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia

Alqahtani, O; Stapleton, P; Gibbons, S; (2023) Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia. Saudi Pharmaceutical Journal , 31 (7) pp. 1237-1243. 10.1016/j.jsps.2023.05.015. Green open access

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Abstract

Seventeen water samples were collected from four different thermal springs in Saudi Arabia. Microbiological assays were used to assess the antibacterial activities of bacterial colonies against antibiotic-resistant and susceptible-bacterial strains, and 16S rRNA gene sequencing was used to identify the genus and species of these antibiotic-producing bacteria. Chromatography and spectroscopy were used to separate the active compounds and help figuring out what their structures were. Four compounds were isolated using bacteria: N-acetyltryptamine (1), isovaleric acid (2), ethyl-4-ethoxybenzoate (3) and phenylacetic acid (4). Compounds 1, 2 and 4 were produced from Bacillus pumilus and 3 was from Bacillus licheniformis (AH-E1). The outcomes of the minimum inhibitory concentrations (MICs) showed that all pure compounds produced in this work had antibacterial activities against Gram-positive pathogens (between 128 mg/L and 512 mg/L compared to the control) and compound 2 had activity against E. coli.

Type: Article
Title: Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia
Location: Saudi Arabia
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.jsps.2023.05.015
Publisher version: https://doi.org/10.1016/j.jsps.2023.05.015
Language: English
Additional information: © 2023 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keywords: 16S rRNA gene sequencing, Antimicrobial agents, Bacillus spp., Bacterial resistance, Thermal springs
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry
URI: https://discovery.ucl.ac.uk/id/eprint/10174413
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