Alqahtani, O;
Stapleton, P;
Gibbons, S;
(2023)
Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia.
Saudi Pharmaceutical Journal
, 31
(7)
pp. 1237-1243.
10.1016/j.jsps.2023.05.015.
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Abstract
Seventeen water samples were collected from four different thermal springs in Saudi Arabia. Microbiological assays were used to assess the antibacterial activities of bacterial colonies against antibiotic-resistant and susceptible-bacterial strains, and 16S rRNA gene sequencing was used to identify the genus and species of these antibiotic-producing bacteria. Chromatography and spectroscopy were used to separate the active compounds and help figuring out what their structures were. Four compounds were isolated using bacteria: N-acetyltryptamine (1), isovaleric acid (2), ethyl-4-ethoxybenzoate (3) and phenylacetic acid (4). Compounds 1, 2 and 4 were produced from Bacillus pumilus and 3 was from Bacillus licheniformis (AH-E1). The outcomes of the minimum inhibitory concentrations (MICs) showed that all pure compounds produced in this work had antibacterial activities against Gram-positive pathogens (between 128 mg/L and 512 mg/L compared to the control) and compound 2 had activity against E. coli.
| Type: | Article |
|---|---|
| Title: | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| Location: | Saudi Arabia |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jsps.2023.05.015 |
| Publisher version: | https://doi.org/10.1016/j.jsps.2023.05.015 |
| Language: | English |
| Additional information: | © 2023 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| Keywords: | 16S rRNA gene sequencing, Antimicrobial agents, Bacillus spp., Bacterial resistance, Thermal springs |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharma and Bio Chemistry |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10174413 |
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