Kopach, Olga;
Sylantyev, Sergyi;
Bard, Lucie;
Michaluk, Piotr;
Heller, Janosch P;
Gutierrez del Arroyo, Ana;
Ackland, Gareth L;
... Rusakov, Dmitri A; + view all
(2023)
Human neutrophils communicate remotely via calcium-dependent glutamate-induced glutamate release.
iScience
, 26
(7)
, Article 107236. 10.1016/j.isci.2023.107236.
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Abstract
Summary Neutrophils are white blood cells that are critical to acute inflammatory and adaptive immune responses. Their swarming-pattern behavior is controlled by multiple cellular cascades involving calcium-dependent release of various signaling molecules. Previous studies have reported that neutrophils express glutamate receptors and can release glutamate but evidence of direct neutrophil-neutrophil communication has been elusive. Here, we hold semi-suspended cultured human neutrophils in patch-clamp whole-cell mode to find that calcium mobilization induced by stimulating one neutrophil can trigger an N-methyl-D-aspartate (NMDA) receptor-driven membrane current and calcium signal in neighboring neutrophils. We employ an enzymatic-based imaging assay to image, in real time, glutamate release from neutrophils induced by glutamate released from their neighbors. These observations provide direct evidence for a positive-feedback inter-neutrophil communication that could contribute to mechanisms regulating communal neutrophil behavior.
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