Engrola, Filipa;
Correia, Márcia AS;
Watson, Cameron;
Romão, Carlos C;
Veiros, Luis F;
Romão, Maria João;
Santos-Silva, Teresa;
(2023)
Arsenite oxidase in complex with antimonite and arsenite oxyanions - insights into the catalytic mechanism.
Journal of Biological Chemistry
, Article 105036. 10.1016/j.jbc.2023.105036.
(In press).
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Abstract
Arsenic contamination of groundwater is among one of the biggest health threats affecting millions of people in the world. There is an urgent need for efficient arsenic biosensors where the use of arsenic metabolizing enzymes can be explored. In this work we have solved the crystal structures of four complexes of arsenite oxidase (Aio) obtained in complex with arsenic and antimony oxyanions and the structures determined correspond to intermediate states of the enzymatic mechanism. These structural data were complemented with DFT calculations providing a unique view of the molybdenum active site at different time points that, together with mutagenesis data, enabled to clarify the enzymatic mechanism and the molecular determinants for the oxidation of As(III) to the less toxic As(V) species.
| Type: | Article |
|---|---|
| Title: | Arsenite oxidase in complex with antimonite and arsenite oxyanions - insights into the catalytic mechanism |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.jbc.2023.105036 |
| Publisher version: | https://doi.org/10.1016/j.jbc.2023.105036 |
| Language: | English |
| Additional information: | This is an Open Access article published under a Creative Commons Attribution 4.0 International (CC BY 4.0) Licence (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | Arsenite oxidase, enzyme mechanism, DFT calculations, X-ray crystallography, molybdenum enzyme, arsenic, antimony |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Structural and Molecular Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10173391 |
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