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Technical transfer of a rapid microbial platform for vaccine production

De Sa Magalhaes, Salome; Morris, Stephen; Kusumawardani, Shinta; Riza, Acep Wijayadikusumah; Nurainy, Neni; Keshavarz-Moore, Eli; (2023) Technical transfer of a rapid microbial platform for vaccine production. Vaccine Insights , 2 (4) pp. 133-146. 10.18609/vac.2023.024. Green open access

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Abstract

The limited availability and affordability of vaccines to low- and middle-income countries (LMIC) has created a need for solutions that will ensure effective, affordable vaccine production technology. To establish a rapid and economical platform for the expression of viral proteins in high yield and purity by Pichia pastoris (X33), the receptor-binding domain (RBD) protein of the SARS-CoV2 was selected in this study. After fermentation at the 5 L scale, the protein was purified by a simplified chromatography, with minimal sample treatment. The purified protein was characterized biochemically, and after its formulation, the immunogenicity was evaluated in mice. Collectively, the data suggested that the vaccine candidate is a suitable COVID-19 vaccine candidate antigen for technology transfer. Furthermore, this study creates a robust foundation for industrial production at scale.

Type: Article
Title: Technical transfer of a rapid microbial platform for vaccine production
Open access status: An open access version is available from UCL Discovery
DOI: 10.18609/vac.2023.024
Publisher version: http://dx.doi.org/10.18609/vac.2023.024
Language: English
Additional information: Copyright: Published by Vaccine Insights under Creative Commons License Deed CC BY NC ND 4.0 which allows anyone to copy, distribute, and transmit the article provided it is properly attributed in the manner specified below. No commercial use without permission. Attribution: Copyright © 2023 Keshavarz-Moore E. Published by Vaccine Insights under Creative Commons License Deed CC BY NC ND 4.0.
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Biochemical Engineering
URI: https://discovery.ucl.ac.uk/id/eprint/10173311
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