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Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish

Chong-Morrison, Vanessa; Mayes, Sarah; Simões, Filipa C; Senanayake, Upeka; Carroll, Dervla S; Riley, Paul R; Wilson, Stephen W; (2023) Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish. Biology Open , 12 (6) , Article bio059995. 10.1242/bio.059995. Green open access

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Abstract

Due to its genetic amenability coupled with advances in genome editing, zebrafish is an excellent model to examine the function of (epi)genomic elements. Here, we repurposed the Ac/Ds maize transposition system to efficiently characterise zebrafish cis-regulated elements, also known as enhancers, in F0-microinjected embryos. We further used the system to stably express guide RNAs enabling CRISPR/dCas9-interference (CRISPRi) perturbation of enhancers without disrupting the underlying genetic sequence. In addition, we probed the phenomenon of antisense transcription at two neural crest gene loci. Our study highlights the utility of Ac/Ds transposition as a new tool for transient epigenome modulation in zebrafish.

Type: Article
Title: Ac/Ds transposition for CRISPR/dCas9-SID4x epigenome modulation in zebrafish
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/bio.059995
Publisher version: https://doi.org/10.1242/bio.059995
Language: English
Additional information: © 2023. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Keywords: Ac/Ds, Antisense, CRISPRi, Enhancer, Zebrafish, Animals, Zebrafish, Clustered Regularly Interspaced Short Palindromic Repeats, Epigenome, Gene Editing
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10172819
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