Alonso, MT;
Torres-Vidal, P;
Calvo, B;
Rodriguez, C;
Delrio-Lorenzo, A;
Rojo-Ruiz, J;
Garcia-Sancho, J;
(2023)
Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles.
Biochimica et Biophysica Acta - Molecular Cell Research
, 1870
(6)
, Article 119481. 10.1016/j.bbamcr.2023.119481.
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Abstract
Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca2+ upon cell activation. Hence, reliable recording of Ca2+ dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca2+ indicators (GECIs) are valuable tools to monitor Ca2+ in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.
| Type: | Article |
|---|---|
| Title: | Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles |
| Location: | Netherlands |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.bbamcr.2023.119481 |
| Publisher version: | https://doi.org/10.1016/j.bbamcr.2023.119481 |
| Language: | English |
| Additional information: | © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). |
| Keywords: | Acidic, Ca(2+) signalling, Endosome, GECI, Genetically encoded calcium indicator, Luminescence, Lysosome, Organelle, Secretory vesicle, Subcellular Ca(2+) |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10171452 |
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