UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles

Alonso, MT; Torres-Vidal, P; Calvo, B; Rodriguez, C; Delrio-Lorenzo, A; Rojo-Ruiz, J; Garcia-Sancho, J; (2023) Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles. Biochimica et Biophysica Acta - Molecular Cell Research , 1870 (6) , Article 119481. 10.1016/j.bbamcr.2023.119481. Green open access

[thumbnail of 1-s2.0-S0167488923000538-main.pdf]
Preview
Text
1-s2.0-S0167488923000538-main.pdf - Published Version

Download (1MB) | Preview

Abstract

Over the last years, there is accumulating evidence that acidic organelles can accumulate and release Ca2+ upon cell activation. Hence, reliable recording of Ca2+ dynamics in these compartments is essential for understanding the physiopathological aspects of acidic organelles. Genetically encoded Ca2+ indicators (GECIs) are valuable tools to monitor Ca2+ in specific locations, although their use in acidic compartments is challenging due to the pH sensitivity of most available fluorescent GECIs. By contrast, bioluminescent GECIs have a combination of features (marginal pH sensitivity, low background, no phototoxicity, no photobleaching, high dynamic range and tunable affinity) that render them advantageous to achieve an enhanced signal-to-noise ratio in acidic compartments. This article reviews the use of bioluminescent aequorin-based GECIs targeted to acidic compartments. A need for more measurements in highly acidic compartments is identified.

Type: Article
Title: Use of aequorin-based indicators for monitoring Ca2+ in acidic organelles
Location: Netherlands
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.bbamcr.2023.119481
Publisher version: https://doi.org/10.1016/j.bbamcr.2023.119481
Language: English
Additional information: © 2023 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
Keywords: Acidic, Ca(2+) signalling, Endosome, GECI, Genetically encoded calcium indicator, Luminescence, Lysosome, Organelle, Secretory vesicle, Subcellular Ca(2+)
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
URI: https://discovery.ucl.ac.uk/id/eprint/10171452
Downloads since deposit
72Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item