UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells

Li, YM; Patel, KD; Han, YK; Hong, SM; Meng, YX; Lee, HH; Park, JH; ... Kim, HW; + view all (2023) Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells. Chemical Engineering Journal , 466 , Article 143125. 10.1016/j.cej.2023.143125.

[thumbnail of Manuscript 230417.pdf] Text
Manuscript 230417.pdf - Accepted Version
Access restricted to UCL open access staff until 16 June 2024.

Download (63MB)

Abstract

Diseased or injured nervous systems lead to lifelong disability and even death due to their limited self-repair and regenerative capability. For successful neuronal regeneration and functional recovery, it is critical to selectively guide neural stem/progenitor cells (NSPCs) to differentiate into neurons and oligodendrocytes rather than astrocytes. Biomaterial scaffolds with specific properties such as electroconductivity can boost this event and guide the specific differentiation of NSPCs. Here, we prepared electroconductive and mechano-competent scaffolds made of shape-memory polyurethane‐polycaprolactone (PUCL) nanofibers and carbon nanotubes (CNTs) (namely, PUCL@CNT). The CNT-decorated PUCL platforms provided excellent shape-formable and shape-memorable properties for surgical compatibility as well as electroconductivity (∼ 8 S/m) and nanoscale-topography (tens-of-nm), which could promote the initial cell adhesion via the integrin-mediated focal adhesion signaling pathway, and promote neuron and oligodendrocyte differentiation. The potential mechanism of this lineage-specific differentiation of NSPCs upon the PUCL@CNT was evaluated by transcriptome sequencing analysis and pharmacological intervention tests, revealing the involvement of the intracellular calcium levels and the FAK-AKT-β-catenin pathway. When implanted in a rat corticectomy model, PUCL@CNT could induce a larger number of endogenous neural stem cells (both Nestin+ and β-catenin+) and neurons (Tuj1+) near the injured region (vs. PUCL and PBS), demonstrating the in vivo therapeutic role of PUCL@CNT. Taken together, the CNT surface-decoration was effective in driving NSPCs toward neuronal specification and their maturation, and the PUCL@CNT scaffold is potentially useful for neural tissue regeneration that can benefit via the neuronal activation of endogenous NSPCs.

Type: Article
Title: Electroconductive and mechano-competent PUCL@CNT nanohybrid scaffolds guiding neuronal specification of neural stem/progenitor cells
DOI: 10.1016/j.cej.2023.143125
Publisher version: http://doi.org/10.1016/j.cej.2023.143125
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Carbon nanotubes, Polyurethane‐polycaprolactone, Neuronal differentiation, Focal adhesion, Intracellular calcium, Neural tissue regeneration
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Eastman Dental Institute > Biomaterials and Tissue Eng
URI: https://discovery.ucl.ac.uk/id/eprint/10170112
Downloads since deposit
1Download
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item