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Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B

Lera-Ramirez, Manuel; Nedelec, Francois J; Tran, Phong T; (2022) Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B. eLife , 11 , Article e72630. 10.7554/eLife.72630. Green open access

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Abstract

During anaphase B, molecular motors slide interpolar microtubules to elongate the mitotic spindle, contributing to the separation of chromosomes. However, sliding of antiparallel microtubules reduces their overlap, which may lead to spindle breakage, unless microtubules grow to compensate sliding. How sliding and growth are coordinated is still poorly understood. In this study, we have used the 1ssion yeast S. pombe to measure microtubule dynamics during anaphase B. We report that the coordination of microtubule growth and sliding relies on promoting rescues at the midzone edges. This makes microtubules stable from pole to midzone, while their distal parts including the plus ends alternate between assembly and disassembly. Consequently, the midzone keeps a constant length throughout anaphase, enabling sustained sliding without the need for a precise regulation of microtubule growth speed. Additionally, we found that in S. pombe, which undergoes closed mitosis, microtubule growth speed decreases when the nuclear 22 membrane wraps around the spindle midzone.

Type: Article
Title: Microtubule rescue at midzone edges promotes overlap stability and prevents spindle collapse during anaphase B
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.7554/eLife.72630
Publisher version: https://doi.org/10.7554/eLife.72630
Language: English
Additional information: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third-party material in this article are included in the Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: Science & Technology, Life Sciences & Biomedicine, Biology, Life Sciences & Biomedicine - Other Topics, microtubule dynamics, anaphase, spindle, microtubule sliding, modelling, live cell imaging, S, pombe, FISSION YEAST, MITOTIC SPINDLE, LENGTH, AURORA, MECHANISM, DYNAMICS, COMPLEX, END, CYTOKINESIS, KINASE
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10169762
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