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Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models

Al-Rekabi, Zeinab; Dondi, Camilla; Faruqui, Nilofar; Siddiqui, Nazia S; Elowsson, Linda; Rissler, Jenny; Kåredal, Monica; ... Shaw, Michael; + view all (2023) Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models. Royal Society Open Science , 10 (4) , Article 221426. 10.1098/rsos.221426. Green open access

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Abstract

Annually, an estimated seven million deaths are linked to exposure to airborne pollutants. Despite extensive epidemiological evidence supporting clear associations between poor air quality and a range of short- and long-term health effects, there are considerable gaps in our understanding of the specific mechanisms by which pollutant exposure induces adverse biological responses at the cellular and tissue levels. The development of more complex, predictive, in vitro respiratory models, including two- and three-dimensional cell cultures, spheroids, organoids and tissue cultures, along with more realistic aerosol exposure systems, offers new opportunities to investigate the cytotoxic effects of airborne particulates under controlled laboratory conditions. Parallel advances in high-resolution microscopy have resulted in a range of in vitro imaging tools capable of visualizing and analysing biological systems across unprecedented scales of length, time and complexity. This article considers state-of-the-art in vitro respiratory models and aerosol exposure systems and how they can be interrogated using high-resolution microscopy techniques to investigate cell–pollutant interactions, from the uptake and trafficking of particles to structural and functional modification of subcellular organelles and cells. These data can provide a mechanistic basis from which to advance our understanding of the health effects of airborne particulate pollution and develop improved mitigation measures.

Type: Article
Title: Uncovering the cytotoxic effects of air pollution with multi-modal imaging of in vitro respiratory models
Open access status: An open access version is available from UCL Discovery
DOI: 10.1098/rsos.221426
Publisher version: https://doi.org/10.1098/rsos.221426
Language: English
Additional information: © 2023 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
Keywords: Respiratory toxicology, bioimaging, microscopy, cell and tissue culture models
UCL classification: UCL
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Engineering Science > Dept of Computer Science
URI: https://discovery.ucl.ac.uk/id/eprint/10168213
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