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A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver

Ceccacci, Elena; Villa, Emanuela; Santoro, Fabio; Minucci, Saverio; Ruhrberg, Christiana; Fantin, Alessandro; (2023) A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver. Journal of Developmental Biology , 11 (2) , Article 15. 10.3390/jdb11020015. Green open access

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Abstract

During prenatal life, the foetal liver is colonised by several waves of haematopoietic progenitors to act as the main haematopoietic organ. Single cell (sc) RNA-seq has been used to identify foetal liver cell types via their transcriptomic signature and to compare gene expression patterns as haematopoietic development proceeds. To obtain a refined single cell landscape of haematopoiesis in the foetal liver, we have generated a scRNA-seq dataset from a whole mouse E12.5 liver that includes a larger number of cells than prior datasets at this stage and was obtained without cell type preselection to include all liver cell populations. We combined mining of this dataset with that of previously published datasets at other developmental stages to follow transcriptional dynamics as well as the cell cycle state of developing haematopoietic lineages. Our findings corroborate several prior reports on the timing of liver colonisation by haematopoietic progenitors and the emergence of differentiated lineages and provide further molecular characterisation of each cell population. Extending these findings, we demonstrate the existence of a foetal intermediate haemoglobin profile in the mouse, similar to that previously identified in humans, and a previously unidentified population of primitive erythroid cells in the foetal liver.

Type: Article
Title: A Refined Single Cell Landscape of Haematopoiesis in the Mouse Foetal Liver
Open access status: An open access version is available from UCL Discovery
DOI: 10.3390/jdb11020015
Publisher version: https://doi.org/10.3390/jdb11020015
Language: English
Additional information: Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
Keywords: foetal liver; haematopoietic development; haemoglobin
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10167754
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