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Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans

Hung, OS; Lee, OO; Thiyagarajan, V; He, HP; Xu, Y; Chung, HC; Qiu, JW; (2009) Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans. Aquatic Biology , 4 (3) pp. 253-262. 10.3354/ab00110. Green open access

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Abstract

Multi-species natural microbial biofilms have been identified as sources of cues to induce larval attachment of a broad range of sessile marine invertebrates. However, the chemical identities of the cues originating from these films have not been fully characterized. In this study, we isolated and characterized 2 compounds from natural biofilms that induced the larval attachment of the polychaete Hydroides elegans. Biofilms were developed on Petri dishes in seawater in the field for 6 d, collected from the Petri dishes using cotton buds, and extracted using a combination of polar and non-polar solvents. The non-polar fraction was inductive to larval attachment and was thus further fractionated using HPLC. Bioassay-guided fractionation was used to locate the active compounds that were then structurally characterized by using nuclear magnetic resonance (NMR) spectroscopy. Two inductive compounds were identified, a hydrocarbon (6,9-heptadecadiene) and a fatty acid (12-octadecenoic acid), which are the first chemical cues inductive to larval attachment of marine invertebrates isolated from natural biofilms.

Type: Article
Title: Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans
Open access status: An open access version is available from UCL Discovery
DOI: 10.3354/ab00110
Publisher version: http://doi.org/10.3354/ab00110
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Inductive cues, Natural biofilms, Hydroides elegans, fatty acid, Hydrocarbon
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10166353
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