Hung, OS;
Lee, OO;
Thiyagarajan, V;
He, HP;
Xu, Y;
Chung, HC;
Qiu, JW;
(2009)
Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans.
Aquatic Biology
, 4
(3)
pp. 253-262.
10.3354/ab00110.
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Abstract
Multi-species natural microbial biofilms have been identified as sources of cues to induce larval attachment of a broad range of sessile marine invertebrates. However, the chemical identities of the cues originating from these films have not been fully characterized. In this study, we isolated and characterized 2 compounds from natural biofilms that induced the larval attachment of the polychaete Hydroides elegans. Biofilms were developed on Petri dishes in seawater in the field for 6 d, collected from the Petri dishes using cotton buds, and extracted using a combination of polar and non-polar solvents. The non-polar fraction was inductive to larval attachment and was thus further fractionated using HPLC. Bioassay-guided fractionation was used to locate the active compounds that were then structurally characterized by using nuclear magnetic resonance (NMR) spectroscopy. Two inductive compounds were identified, a hydrocarbon (6,9-heptadecadiene) and a fatty acid (12-octadecenoic acid), which are the first chemical cues inductive to larval attachment of marine invertebrates isolated from natural biofilms.
Type: | Article |
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Title: | Characterization of cues from natural multi-species biofilms that induce larval attachment of the polychaete Hydroides elegans |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.3354/ab00110 |
Publisher version: | http://doi.org/10.3354/ab00110 |
Language: | English |
Additional information: | This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions. |
Keywords: | Inductive cues, Natural biofilms, Hydroides elegans, fatty acid, Hydrocarbon |
UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology |
URI: | https://discovery.ucl.ac.uk/id/eprint/10166353 |
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