UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell lines

Sevrin, T; Strasser, L; Ternet, C; Junk, P; Caffarini, M; Prins, S; D'Arcy, C; ... Luthert, PJ; + view all (2023) Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell lines. iScience , 26 (2) , Article 105931. 10.1016/j.isci.2023.105931. Green open access

[thumbnail of 1-s2.0-S2589004223000081-main.pdf]
Preview
Text
1-s2.0-S2589004223000081-main.pdf - Published Version

Download (3MB) | Preview

Abstract

Cellular utilization of available energy flows to drive a multitude of forms of cellular “work” is a major biological constraint. Cells steer metabolism to address changing phenotypic states but little is known as to how bioenergetics couples to the richness of processes in a cell as a whole. Here, we outline a whole-cell energy framework that is informed by proteomic analysis and an energetics-based gene ontology. We separate analysis of metabolic supply and the capacity to generate high-energy phosphates from a representation of demand that is built on the relative abundance of ATPases and GTPases that deliver cellular work. We employed mouse embryonic fibroblast cell lines that express wild-type KRAS or oncogenic mutations and with distinct phenotypes. We observe shifts between energy-requiring processes. Calibrating against Seahorse analysis, we have created a whole-cell energy budget with apparent predictive power, for instance in relation to protein synthesis.

Type: Article
Title: Whole-cell energy modeling reveals quantitative changes of predicted energy flows in RAS mutant cancer cell lines
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.isci.2023.105931
Publisher version: https://doi.org/10.1016/j.isci.2023.105931
Language: English
Additional information: © 2023 This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Keywords: Cellular physiology, Protein
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology
URI: https://discovery.ucl.ac.uk/id/eprint/10165072
Downloads since deposit
25Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item