Zattera, Elena;
(2023)
Development of gold nanosensors for the detection of metal
ions as a diagnostic tool for implant failure.
Doctoral thesis (Ph.D), UCL (University College London).
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Abstract
Although the use of metallic implants has improved the quality of life of millions of individuals, the release of debris and toxic metal ions, such as chromium, from these implants (e.g. MoM hip implants) through wear into the physiological environment can lead to localised toxicity and widespread systemic effects associated with the failure of the medical device. This project aimed at developing an easy-to-use colourimetric technology based on gold nanoparticles (AuNPs), which can detect levels of chromium within the threshold range imposed by different worldwide health authorities (e.g. MHRA 7 ppb). The detection of different chromium compounds was first tested with citrated AuNPs, investigating the effect of different parameters on the detection (ion concentration, AuNP size and concentration, and time of detection). AuNPs were then modified with PEG thiols to increase their stability in physiological solutions and finally with carboxylated compounds to improve their detection. While the interaction of citrated AuNPs with Cr led to the aggregation of AuNPs, characterised by a change in colour of the solution from pink to grey, in the case of modified AuNPs, this interaction led to a decrease in the colour intensity of the solution measured by eye inspection and UV-vis. Once all parameters were optimised, an AuNP formulation based on methyl PEG and GSH with optimised pH and AuNP concentration led to a LOD of 5, 40 and 70 ppb of Cr as CrCl2, CrCl3·6H2O and CrO3 in water after 24 hours, displaying the potential to become a diagnostic test for detection of chromium in human samples. Three of the developed AuNP formulations also demonstrated colourimetric detection of iron within the range found in human blood (600-2000 -ppb) in just 10 minutes. These could be used for the detection of iron in human samples.
| Type: | Thesis (Doctoral) |
|---|---|
| Qualification: | Ph.D |
| Title: | Development of gold nanosensors for the detection of metal ions as a diagnostic tool for implant failure |
| Open access status: | An open access version is available from UCL Discovery |
| Language: | English |
| Additional information: | Copyright © The Author 2022. Original content in this thesis is licensed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0) Licence (https://creativecommons.org/licenses/by-nc/4.0/). Any third-party copyright material present remains the property of its respective owner(s) and is licensed under its existing terms. Access may initially be restricted at the author’s request. |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Surgical Biotechnology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Surgery and Interventional Sci > Department of Targeted Intervention UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10164402 |
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