He, Peng;
Lim, Kyungtae;
Sun, Dawei;
Pett, Jan Patrick;
Jeng, Quitz;
Polanski, Krzysztof;
Dong, Ziqi;
... Rawlins, Emma L; + view all
(2022)
A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates.
Cell
, 185
(25)
4841-4860.e25.
10.1016/j.cell.2022.11.005.
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Abstract
We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.
| Type: | Article |
|---|---|
| Title: | A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.cell.2022.11.005 |
| Publisher version: | https://doi.org/10.1016/j.cell.2022.11.005 |
| Language: | English |
| Additional information: | © 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Respiratory Medicine |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10162081 |
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