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Genome-wide association mapping of Hagberg falling number, protein content, test weight, and grain yield in UK wheat

White, Jon; Sharma, Rajiv; Balding, David; Cockram, James; Mackay, Ian J; (2022) Genome-wide association mapping of Hagberg falling number, protein content, test weight, and grain yield in UK wheat. Crop Science , 62 (3) pp. 965-981. 10.1002/csc2.20692. Green open access

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Abstract

Association mapping using crop cultivars allows identification of genetic loci of direct relevance to breeding. Here, 150 U.K. wheat (Triticum aestivum L.) cultivars genotyped with 23,288 single nucleotide polymorphisms (SNPs) were used for genome-wide association studies (GWAS) using historical phenotypic data for grain protein content, Hagberg falling number (HFN), test weight, and grain yield. Power calculations indicated experimental design would enable detection of quantitative trait loci (QTL) explaining ≥20% of the variation (PVE) at a relatively high power of >80%, falling to 40% for detection of a SNP with an R2≥.5 with the same QTL. Genome-wide association studies identified marker-trait associations for all four traits. For HFN (h2=.89), six QTL were identified, including a major locus on chromosome 7B explaining 49% PVE and reducing HFN by 44 s. For protein content (h2= 0.86), 10 QTL were found on chromosomes 1A, 2A, 2B, 3A, 3B, and 6B, together explaining 48.9% PVE. For test weight, five QTL were identified (one on 1B and four on 3B; 26.3% PVE). Finally, 14 loci were identified for grain yield (h2= 0.95) on eight chromosomes (1A, 2A, 2B, 2D, 3A, 5B, 6A, 6B; 68.1% PVE), of which five were located within 16 Mbp of genetic regions previously identified as under breeder selection in European wheat. Our study demonstrates the utility of exploiting historical crop datasets, identifying genomic targets for independent validation, and ultimately for wheat genetic improvement.

Type: Article
Title: Genome-wide association mapping of Hagberg falling number, protein content, test weight, and grain yield in UK wheat
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1002/csc2.20692
Publisher version: https://doi.org/10.1002/csc2.20692
Language: English
Additional information: © 2022 The Authors. Crop Science published by Wiley Periodicals LLC on behalf of Crop Science Society of America. This is an open access article under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
URI: https://discovery.ucl.ac.uk/id/eprint/10161941
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