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The association between vitamin D receptor polymorphism and phases of chronic hepatitis B infection in HBV carriers in Thailand

Ananchuensook, Prooksa; Suksawatamnauy, Sirinporn; Thaimai, Panarat; Sriphoosanaphan, Supachaya; Thanapirom, Kessarin; Teerapakpinyo, Chinachote; Pooworawan, Yong; (2022) The association between vitamin D receptor polymorphism and phases of chronic hepatitis B infection in HBV carriers in Thailand. PLoS One , 17 (12) , Article e0277907. 10.1371/journal.pone.0277907. Green open access

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Abstract

Vitamin D receptor (VDR) polymorphism partly regulates the immune system and is associated with hepatic flare in chronic Hepatitis B virus infection (HBV). Our study identified the association between two distinct phases, VDR polymorphisms and HBV inactive carrier (IC) and chronic hepatitis (CH). Chronic HBV patients were enrolled from February to August 2020. An HBV viral load (VL) < 2,000 IU/ml twice for 6 months apart, with no prior history of HBV treatment, defined the IC phase. Six common polymorphisms in the VDR gene, including CdX-2, GATA, FokI, Bsml, ApaI, and TaqI, were studied using real-time PCR. The different outcomes in allele, genotype, and haplotype frequencies in between groups and linkage disequilibrium (LD) mapping were analyzed. Among 324 enrolled patients, there were 163 patients in IC and 161 patients in CH phases. The mean vitamin D levels were not statistically different between groups. The proportion of allele frequencies of CdX-2 in IC and CH was 53.7% and 62.7% for G allele, and 46.3% and 37.3% for A allele (p 0.019). The proportion of GG genotype of CdX-2 was less frequently found in patients with IC compared to that in patients with CH (27% vs 41%, p 0.028). By multivariate analysis, CdX-2 G/A genotypes were independently associated with IC, with adjusted odd ratio (OR) 1.83 (1.10-3.04), p 0.019. The LD mapping of single nucleotide polymorphisms (SNP) revealed high LD scores in Bsml/ApaI/TaqI (BAT) haplotype in both groups while, CdX-2/GATA and GATA/FokI demonstrated high LD score only in CH group. CdX-2 G/A genotypes were independently associated with IC status in Thai patients with chronic HBV infection. The difference in LD of the CdX-2/GATA and GATA/FokI haplotypes in between groups may represent a non-random selection resulting in the variation of immune control.

Type: Article
Title: The association between vitamin D receptor polymorphism and phases of chronic hepatitis B infection in HBV carriers in Thailand
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.pone.0277907
Publisher version: https://doi.org/10.1371/journal.pone.0277907
Language: English
Additional information: © 2022 Ananchuensook et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Keywords: Humans, Receptors, Calcitriol, Hepatitis B, Chronic, Polymorphism, Single Nucleotide, Gene Frequency, Alleles, Genotype, Haplotypes, Genetic Predisposition to Disease, Case-Control Studies
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10161767
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