Kellaway, Simon C;
Roberton, Victoria;
Jones, Joshua N;
Loczenski, Rabea;
Phillips, James B;
White, Lisa J;
(2022)
Engineered neural tissue made using hydrogels derived from decellularised tissues for the regeneration of peripheral nerves.
Acta Biomaterialia
10.1016/j.actbio.2022.12.003.
(In press).
Preview |
PDF
Roberton_Engineered neural tissue made using hydrogels derived from decellularised tissues for the regeneration of peripheral nerves_AOP.pdf - Published Version Download (1MB) | Preview |
Abstract
Engineered neural tissue (EngNT) promotes in vivo axonal regeneration. Decellularised materials (dECM) are complex biologic scaffolds that can improve the cellular environment and also encourage positive tissue remodelling in vivo. We hypothesised that we could incorporate a hydrogel derived from a decellularised tissue (dECMh) into EngNT, thereby providing an alternative to the currently used purified collagen I hydrogel for the first time. Decellularisation was carried out on bone (B-ECM), liver (LIV-ECM), and small intestinal (SIS-ECM) tissues and the resultant dECM was biochemically and mechanically characterised. dECMh differed in mechanical and biochemical properties that likely had an effect on Schwann cell behaviour observed in metabolic activity and contraction profiles. Cellular alignment was observed in tethered moulds within the B-ECM and SIS-ECM derived hydrogels only. No difference was observed in dorsal root ganglia (DRG) neurite extension between the dECMh groups and collagen I groups when applied as a coverslip coating, however, when DRG were seeded atop EngNT constructs, only the B-ECM derived EngNT performed similarly to collagen I derived EngNT. B-ECM EngNT further exhibited similar axonal regeneration to collagen I EngNT in a 10 mm gap rat sciatic nerve injury model after 4 weeks. Our results have shown that various dECMh can be utilised to produce EngNT that can promote neurite extension in vitro and axonal regeneration in vivo.
| Type: | Article |
|---|---|
| Title: | Engineered neural tissue made using hydrogels derived from decellularised tissues for the regeneration of peripheral nerves |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1016/j.actbio.2022.12.003 |
| Publisher version: | https://doi.org/10.1016/j.actbio.2022.12.003 |
| Language: | English |
| Additional information: | © 2022 Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/4.0/) |
| Keywords: | Decellularised ECM, peripheral nerve regeneration, peripheral nerve repair |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > UCL School of Pharmacy > Pharmacology |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10161644 |
Archive Staff Only
 |
View Item |
