Hill, Emily;
Moffat, Kevin G;
Wall, Mark J;
Zetterberg, Henrik;
Blennow, Kaj;
Karikari, Thomas K;
(2022)
A Validated Method to Prepare Stable Tau Oligomers.
Protein Aggregation: Methods in Molecular Biology
, 2551
pp. 203-224.
10.1007/978-1-0716-2597-2_14.
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Abstract
There is growing evidence that tau oligomers are a major pathological species in a number of tauopathies including Alzheimer's disease. However, it is still unclear what exact mechanisms underlie tau oligomer-mediated dysfunction. Studies of tau oligomers in vitro are limited by the high propensity for aggregation and consequent changes in the aggregation state of the produced tau samples over time. In this protocol, we provide a step-by-step description of a validated method for producing stable and structurally characterized oligomers of tau that can be used in biochemical, cellular, and animal model studies to evaluate mechanisms of action of tau in tauopathies.
| Type: | Article |
|---|---|
| Title: | A Validated Method to Prepare Stable Tau Oligomers |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2597-2_14 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2597-2_14 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Alzheimer Disease, Animals, Disease Models, Animal, tau Proteins, Tauopathies, Alzheimer’s disease, Oligomer, Protein expression and purification, Stable oligomer, Tau, Tauopathies |
| UCL classification: | UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Neurodegenerative Diseases |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10161428 |
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