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Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo

Stuckey, Daniel W; Clements, Melanie; Di-Gregorio, Aida; Senner, Claire E; Le Tissier, Paul; Srinivas, Shankar; Rodriguez, Tristan A; (2011) Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo. Development , 138 (8) pp. 1521-1530. 10.1242/dev.063537. Green open access

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Abstract

During development, the growth of the embryo must be coupled to its patterning to ensure correct and timely morphogenesis. In the mouse embryo, migration of the anterior visceral endoderm (AVE) to the prospective anterior establishes the anteriorposterior (A-P) axis. By analysing the distribution of cells in S phase, M phase and G2 from the time just prior to the migration of the AVE until 18 hours after its movement, we show that there is no evidence for differential proliferation along the A-P axis of the mouse embryo. Rather, we have identified that as AVE movements are being initiated, the epiblast proliferates at a much higher rate than the visceral endoderm. We show that these high levels of proliferation in the epiblast are dependent on Nodal signalling and are required for A-P establishment, as blocking cell division in the epiblast inhibits AVE migration. Interestingly, inhibition of migration by blocking proliferation can be rescued by Dkk1. This suggests that the high levels of epiblast proliferation function to move the prospective AVE away from signals that are inhibitory to its migration. The finding that initiation of AVE movements requires a certain level of proliferation in the epiblast provides a mechanism whereby A-P axis development is coordinated with embryonic growth.

Type: Article
Title: Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo
Location: England
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/dev.063537
Publisher version: https://doi.org/10.1242/dev.063537
Language: English
Additional information: This version is the version of record. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Anterior-posterior axis establishment, Nodal signalling, Mouse embryo, Proliferation, Anterior visceral endoderm
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Cancer Institute > Research Department of Cancer Bio
URI: https://discovery.ucl.ac.uk/id/eprint/10159945
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