Hopkins, Jack;
Asada, Ken;
Leung, Alex;
Papadaki, Vasiliki;
Davaapil, Hongorzul;
Morrison, Matthew;
Orita, Tomoko;
... Ohnuma, Shin-Ichi; + view all
(2022)
PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression.
Cancers
, 14
(19)
, Article 4926. 10.3390/cancers14194926.
Preview |
Text
Sagoo_PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression_VoR.pdf - Published Version Download (4MB) | Preview |
Abstract
Retinoblastoma (RB) is the most common intraocular pediatric cancer. Nearly all cases of RB are associated with mutations compromising the function of the RB1 tumor suppressor gene. We previously demonstrated that PRELP is widely downregulated in various cancers and our in vivo and in vitro analysis revealed PRELP as a novel tumor suppressor and regulator of EMT. In addition, PRELP is located at chromosome 1q31.1, around a region hypothesized to be associated with the initiation of malignancy in RB. Therefore, in this study, we investigated the role of PRELP in RB through in vitro analysis and next-generation sequencing. Immunostaining revealed that PRELP is expressed in Müller glial cells in the retina. mRNA expression profiling of PRELP-/- mouse retina and PRELP-treated RB cells found that PRELP contributes to RB progression via regulation of the cancer microenvironment, in which loss of PRELP reduces cell-cell adhesion and facilitates EMT. Our observations suggest that PRELP may have potential as a new strategy for RB treatment.
| Type: | Article |
|---|---|
| Title: | PRELP Regulates Cell-Cell Adhesion and EMT and Inhibits Retinoblastoma Progression |
| Location: | Switzerland |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.3390/cancers14194926 |
| Publisher version: | https://doi.org/10.3390/cancers14194926 |
| Language: | English |
| Additional information: | © 2022 MDPI. This is an open access article distributed under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). |
| Keywords: | EMT/MET, PRELP, cell–cell adhesion, dysplasia, retinoblastoma |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > Institute of Ophthalmology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10157524 |
Archive Staff Only
 |
View Item |
