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SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice

El-Kafrawy, SA; Odle, A; Abbas, AT; Hassan, AM; Abdel-Dayem, UA; Qureshi, AK; Wong, LYR; ... Azhar, EI; + view all (2022) SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice. PLoS Pathogens , 18 (9) , Article e1010782. 10.1371/journal.ppat.1010782. Green open access

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Abstract

Safe, passive immunization methods are required against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants. Immunization of chickens with antigen is known to induce specific IgY antibodies concentrated in the egg yolk and has a good safety profile, high yield of IgY per egg, can be topically applied, not requiring parenteral delivery. Our data provide the first evidence of the prophylactic efficacy of Immunoglobulin Y antibodies against SARS-CoV-2 in mice. Lohmann hens were injected with recombinant SARS-CoV-2 RBD protein; IgY-Abs were extracted from the eggs and characterized using SDS-PAGE. Antiviral activity was evaluated using plaque reduction neutralization tests. In additional experiments, IgY-RBD efficacy was examined in mice sensitized to SARS-CoV-2 infection by transduction with Ad5-hACE2 (mild disease) or by using mouse-adapted virus (severe disease). In both cases, prophylactic intranasal administration of IgY-Abs reduced SARS-CoV-2 replication, and reduced morbidity, inflammatory cell infiltration, hemorrhage, and edema in the lungs and increased survival compared to control groups that received non-specific IgY-Abs. These results indicate that further evaluation of IgY-RBD antibodies in humans is warranted.

Type: Article
Title: SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1371/journal.ppat.1010782
Publisher version: https://doi.org/10.1371/journal.ppat.1010782
Language: English
Additional information: © 2022 El-Kafrawy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/).
Keywords: Animals, Antibodies, Viral, Antiviral Agents, COVID-19, Chickens, Female, Humans, Immunoglobulins, Mice, SARS-CoV-2
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10157418
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