El-Kafrawy, SA;
Odle, A;
Abbas, AT;
Hassan, AM;
Abdel-Dayem, UA;
Qureshi, AK;
Wong, LYR;
... Azhar, EI; + view all
(2022)
SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice.
PLoS Pathogens
, 18
(9)
, Article e1010782. 10.1371/journal.ppat.1010782.
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Abstract
Safe, passive immunization methods are required against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants. Immunization of chickens with antigen is known to induce specific IgY antibodies concentrated in the egg yolk and has a good safety profile, high yield of IgY per egg, can be topically applied, not requiring parenteral delivery. Our data provide the first evidence of the prophylactic efficacy of Immunoglobulin Y antibodies against SARS-CoV-2 in mice. Lohmann hens were injected with recombinant SARS-CoV-2 RBD protein; IgY-Abs were extracted from the eggs and characterized using SDS-PAGE. Antiviral activity was evaluated using plaque reduction neutralization tests. In additional experiments, IgY-RBD efficacy was examined in mice sensitized to SARS-CoV-2 infection by transduction with Ad5-hACE2 (mild disease) or by using mouse-adapted virus (severe disease). In both cases, prophylactic intranasal administration of IgY-Abs reduced SARS-CoV-2 replication, and reduced morbidity, inflammatory cell infiltration, hemorrhage, and edema in the lungs and increased survival compared to control groups that received non-specific IgY-Abs. These results indicate that further evaluation of IgY-RBD antibodies in humans is warranted.
Type: | Article |
---|---|
Title: | SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice |
Location: | United States |
Open access status: | An open access version is available from UCL Discovery |
DOI: | 10.1371/journal.ppat.1010782 |
Publisher version: | https://doi.org/10.1371/journal.ppat.1010782 |
Language: | English |
Additional information: | © 2022 El-Kafrawy et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Animals, Antibodies, Viral, Antiviral Agents, COVID-19, Chickens, Female, Humans, Immunoglobulins, Mice, SARS-CoV-2 |
UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Infection and Immunity UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL |
URI: | https://discovery.ucl.ac.uk/id/eprint/10157418 |
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