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Reaction-Diffusion Patterning of DNA-Based Artificial Cells

Leathers, Adrian; Walczak, Michal; Brady, Ryan A; Al Samad, Assala; Kotar, Jurij; Booth, Michael J; Cicuta, Pietro; (2022) Reaction-Diffusion Patterning of DNA-Based Artificial Cells. Journal of the American Chemical Society , 144 pp. 17468-17476. 10.1021/jacs.2c06140. Green open access

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Abstract

Biological cells display complex internal architectures with distinct micro environments that establish the chemical heterogeneity needed to sustain cellular functions. The continued efforts to create advanced cell mimics, namely, artificial cells, demands strategies for constructing similarly heterogeneous structures with localized functionalities. Here, we introduce a platform for constructing membraneless artificial cells from the self-assembly of synthetic DNA nanostructures in which internal domains can be established thanks to prescribed reaction-diffusion waves. The method, rationalized through numerical modeling, enables the formation of up to five distinct concentric environments in which functional moieties can be localized. As a proof-of-concept, we apply this platform to build DNA-based artificial cells in which a prototypical nucleus synthesizes fluorescent RNA aptamers that then accumulate in a surrounding storage shell, thus demonstrating the spatial segregation of functionalities reminiscent of that observed in biological cells.

Type: Article
Title: Reaction-Diffusion Patterning of DNA-Based Artificial Cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1021/jacs.2c06140
Publisher version: https://doi.org/10.1021/jacs.2c06140
Language: English
Additional information: Copyright © 2022 The Authors. Published by American Chemical Society. This is an open access article under the CC BY 4.0 license Attribution 4.0 International (https://creativecommons.org/licenses/by/4.0/)
UCL classification: UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences
UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > Dept of Chemistry
UCL > Provost and Vice Provost Offices > UCL BEAMS
UCL
URI: https://discovery.ucl.ac.uk/id/eprint/10156424
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