Xu, Lizhou;
Ramadan, Sami;
Rosa, Bruno Gil;
Zhang, Yuanzhou;
Yin, Tianyi;
Torres, Elias;
Shaforost, Olena;
... Klein, Norbert; + view all
(2022)
On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium.
Sensors & Diagnostics
, 1
(4)
pp. 719-730.
10.1039/d2sd00076h.
Preview |
Text
Li_On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium_VoR.pdf - Published Version Download (5MB) | Preview |
Abstract
Graphene field-effect transistor (GFET) biosensors exhibit high sensitivity due to a large surface-to-volume ratio and the high sensitivity of the Fermi level to the presence of charged biomolecules near the surface. For most reported GFET biosensors, bulky external reference electrodes are used which prevent their full-scale chip integration and contribute to higher costs per test. In this study, GFET arrays with on-chip integrated liquid electrodes were employed for COVID-19 detection and functionalized with either antibody or aptamer to selectively bind the spike proteins of SARS-CoV-2. In the case of the aptamer-functionalized GFET (aptasensor, Apt-GFET), the limit-of-detection (LOD) achieved was about 103 particles per mL for virus-like particles (VLPs) in clinical transport medium, outperforming the Ab-GFET biosensor counterpart. In addition, the aptasensor achieved a LOD of 160 aM for COVID-19 neutralizing antibodies in serum. The sensors were found to be highly selective, fast (sample-to-result within minutes), and stable (low device-to-device signal variation; relative standard deviations below 0.5%). A home-built portable readout electronic unit was employed for simultaneous real-time measurements of 12 GFETs per chip. Our successful demonstration of a portable GFET biosensing platform has high potential for infectious disease detection and other health-care applications.
| Type: | Article |
|---|---|
| Title: | On-chip integrated graphene aptasensor with portable readout for fast and label-free COVID-19 detection in virus transport medium |
| Location: | England |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1039/d2sd00076h |
| Publisher version: | https://doi.org/10.1039/D2SD00076H |
| Language: | English |
| Additional information: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
| UCL classification: | UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office > Institute for Materials Discovery UCL > Provost and Vice Provost Offices > UCL BEAMS UCL UCL > Provost and Vice Provost Offices > UCL BEAMS > Faculty of Maths and Physical Sciences > MAPS Faculty Office |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10154194 |
Archive Staff Only
 |
View Item |
