Chung, Gary Hong Chun;
Lorvellec, Maëlle;
Gissen, Paul;
Pichaud, Franck;
Burden, Jemima J;
Stefan, Christopher J;
(2022)
The ultrastructural organization of endoplasmic reticulum-plasma membrane contacts is conserved in epithelial cells.
Molecular Biology of the Cell
10.1091/mbc.E21-11-0534-T.
(In press).
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Abstract
Contacts between the endoplasmic reticulum and plasma membrane (ER-PM contacts) have important roles in membrane lipid and calcium dynamics. Yet, their organization in polarized epithelial cells has not been thoroughly described. Here, we examine ER-PM contacts in hepatocytes in mouse liver using electron microscopy, providing the first comprehensive ultrastructural study of ER-PM contacts in a mammalian epithelial tissue. Our quantitative analyses reveal strikingly distinct ER-PM contact architectures spatially linked to apical, lateral, and basal PM domains. Notably, we find that an extensive network of ER-PM contacts exists at lateral PM domains that form inter-cellular junctions between hepatocytes. Moreover, the spatial organization of ER-PM contacts is conserved in epithelial spheroids, suggesting that ER-PM contacts may serve conserved roles in epithelial cell architecture. Consistent with this notion, we show that ORP5 activity at ER-PM contacts modulates apical-basolateral aspect ratio in HepG2 cells. Thus, ER-PM contacts have a conserved distribution and crucial roles in PM domain architecture across epithelial cell types. [Media: see text] [Media: see text] [Media: see text] [Media: see text] [Media: see text].
| Type: | Article |
|---|---|
| Title: | The ultrastructural organization of endoplasmic reticulum-plasma membrane contacts is conserved in epithelial cells |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1091/mbc.E21-11-0534-T |
| Publisher version: | https://doi.org/10.1091/mbc.E21-11-0534-T |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | apical domain, basal domain, bile canaliculi, cell-cell junctions, endoplasmic reticulum-plasma membrane contacts, epithelial cells, hepatocytes, lateral domain, xysterolbinding protein related proteins |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Lab for Molecular Cell Bio MRC-UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Genetics and Genomic Medicine Dept UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10153951 |
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