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The uncoating of EV71 in mature late endosomes requires CD-M6PR

Ohka, Seii; Tan, Soon Hao; Ishiyama, Eri; Ogasawara, Katsutoshi; Hanasaka, Tomohito; Ishida, Kinji; Hagiwara, Kyoji; ... Schiavo, Giampietro; + view all (2022) The uncoating of EV71 in mature late endosomes requires CD-M6PR. Biology Open 10.1242/bio.059469. (In press). Green open access

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Abstract

Enterovirus 71 (EV71) is one of the causative agents of hand-foot-and-mouth disease, which in some circumstances could lead to severe neurological diseases. Despite of its importance for human health, little is known about the early stages of EV71 infection. EV71 starts uncoating with its receptor, human scavenger receptor B2 (hSCARB2), at low pH. We show that EV71 was not targeted to lysosomes in human rhabdomyosarcoma cells overexpressing hSCARB2 and that the autophagic pathway is not essential for EV71 productive uncoating. Instead, EV71 was efficiently uncoated 30 minutes after infection in late endosomes (LEs) containing hSCARB2, mannose-6-phosphate receptor (M6PR), RAB9, bis(monoacylglycero)phosphate and lysosomal associated membrane protein 2 (LAMP2). Furthering the notion that mature LEs are crucial for EV71 uncoating, cation-dependent (CD)-M6PR knockdown impairs EV71 infection. Since hSCARB2 interacts with cation-independent (CI)-M6PR through M6P-binding sites and CD-M6PR also harbor a M6P-binding site, CD-M6PR is likely to play important roles in EV71 uncoating in LEs.

Type: Article
Title: The uncoating of EV71 in mature late endosomes requires CD-M6PR
Open access status: An open access version is available from UCL Discovery
DOI: 10.1242/bio.059469
Publisher version: https://doi.org/10.1242/bio.059469
Language: English
Additional information: This version is the author accepted manuscript . For information on re-use, please refer to the publisher’s terms and conditions.
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology > Department of Neuromuscular Diseases
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Brain Sciences > UCL Queen Square Institute of Neurology
URI: https://discovery.ucl.ac.uk/id/eprint/10153412
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