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Alternative cost-effective media to facilitate MODS culture for diagnostics of tuberculosis

Sheen, Patricia; Rodriguez, Joseline; Alcántara, Roberto; Vargas, Johnny; Grandjean, Louis; Moore, David AJ; Gilman, Robert H; (2022) Alternative cost-effective media to facilitate MODS culture for diagnostics of tuberculosis. Tuberculosis , 135 , Article 102225. 10.1016/j.tube.2022.102225. Green open access

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Abstract

Most culture-based methods for tuberculosis diagnosis remain low-cost options for low- and mid-income countries. The MODS culture is a rapid and low-cost assay to diagnose tuberculosis and determine drug susceptibility. However, its implementation is limited due to the low accessibility to supplies required for the enriched medium. In this study, we evaluate two alternative culture media: A powder-based mixed (PM) and a lyophilized media (LM). Catalase, PANTA, and gamma irradiation were evaluated as additions to PM and LM. The culture performance of the alternative media was compared with the standard MODS medium (MM) using Mycobacterium tuberculosis isolates and positive acid-fast smear sputum samples. Overall, no significant difference was observed in the bacterial growth between PM and LM with MM. However, PANTA and gamma irradiation combined reduced bacterial growth significantly in all media variants. A median positivity day of 6 ± 5 days was observed for sputum samples, regardless of the culture medium. The preliminary results show that the two variants culture media have a similar performance to the standard MODS medium. The powder-based media with PANTA (PM_P) showed a time-to-positivity and sensitivity similar to the standard MODS medium. It is the simplest to prepare and does not require any sterilization process.

Type: Article
Title: Alternative cost-effective media to facilitate MODS culture for diagnostics of tuberculosis
Location: Scotland
Open access status: An open access version is available from UCL Discovery
DOI: 10.1016/j.tube.2022.102225
Publisher version: https://doi.org/10.1016/j.tube.2022.102225
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Culture media, Diagnostics, Irradiation, Lyophilization, MODS, Tuberculosis
UCL classification: UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health > Infection, Immunity and Inflammation Dept
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Population Health Sciences > UCL GOS Institute of Child Health
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
URI: https://discovery.ucl.ac.uk/id/eprint/10152588
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