UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Downregulation of CYP17A1 by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties

Aljaber, Maneera Y; Orie, Nelson N; Raees, Asmaa; Kraiem, Suhail; Al-Jaber, Mashael; Samsam, Waseem; Hamza, Mostafa M; ... Almaadheed, Mohammed; + view all (2022) Downregulation of CYP17A1 by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties. Future Science OA 10.2144/fsoa-2022-0006. (In press). Green open access

[thumbnail of Orie_Downregulation of CYP17A1 by 20-hydroxyecdysone_AOP.pdf]
Preview
Text
Orie_Downregulation of CYP17A1 by 20-hydroxyecdysone_AOP.pdf - Published Version

Download (2MB) | Preview

Abstract

Aim: To investigate the effect of 20-hydroxyecdysone on steroidogenic pathway genes and plasma progesterone, and its potential impact on vascular functions. Methods: Chimeric mice with humanized liver were treated with 20-hydroxyecdysone for 3 days, and hepatic steroidogenic pathway genes and plasma progesterone were measured by transcriptomics and GC–MS/MS, respectively. Direct effects on muscle and mesenteric arterioles were assessed by myography. Results: CYP17A1 was downregulated in 20-hydroxyecdysone-treated mice compared with untreated group (p = 0.04), with an insignificant increase in plasma progesterone. Progesterone caused vasorelaxation which was blocked by 60 mM KCl, but unaffected by nitric oxide synthase inhibition. Conclusion: In the short term, 20-hydroxyecdysone mediates CYP17A1 downregulation without a significant increase in plasma progesterone, which has a vasodilatory effect involving inhibition of voltage-dependent calcium channels, and the potential to enhance 20-hydroxyecdysone vasorelaxation.

Type: Article
Title: Downregulation of CYP17A1 by 20-hydroxyecdysone: plasma progesterone and its vasodilatory properties
Open access status: An open access version is available from UCL Discovery
DOI: 10.2144/fsoa-2022-0006
Publisher version: https://doi.org/10.2144/fsoa-2022-0006
Language: English
Additional information: This work is licensed under the Creative Commons Attribution 4.0 License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Keywords: 20-hydroxyecdysone • CYP17A1 • ecdysteroid • hepatic transcriptome • humanized liver • mesenteric arteriole • muscle arteriole • progesterone • vasodilation • voltage-dependent calcium entry
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine > Inflammation
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Medical Sciences > Div of Medicine
URI: https://discovery.ucl.ac.uk/id/eprint/10152365
Downloads since deposit
24Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item