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Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Murphy, MP; Bayir, H; Belousov, V; Chang, CJ; Davies, KJA; Davies, MJ; Dick, TP; ... Halliwell, B; + view all (2022) Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo. Nature Metabolism , 4 (6) pp. 651-662. 10.1038/s42255-022-00591-z. Green open access

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Abstract

Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.

Type: Article
Title: Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo
Location: Germany
Open access status: An open access version is available from UCL Discovery
DOI: 10.1038/s42255-022-00591-z
Publisher version: https://doi.org/10.1038/s42255-022-00591-z
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism, HYDROGEN-PEROXIDE PRODUCTION, LIPID-PEROXIDATION, URINARY F-2-ISOPROSTANES, FLUORESCENT-PROBES, MASS-SPECTROMETRY, SUPEROXIDE ANION, REDOX REGULATION, QUANTIFICATION, OXIDANT, MARKER
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10152301
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