Murphy, MP;
Bayir, H;
Belousov, V;
Chang, CJ;
Davies, KJA;
Davies, MJ;
Dick, TP;
... Halliwell, B; + view all
(2022)
Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo.
Nature Metabolism
, 4
(6)
pp. 651-662.
10.1038/s42255-022-00591-z.
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Abstract
Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.
| Type: | Article |
|---|---|
| Title: | Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo |
| Location: | Germany |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1038/s42255-022-00591-z |
| Publisher version: | https://doi.org/10.1038/s42255-022-00591-z |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Science & Technology, Life Sciences & Biomedicine, Endocrinology & Metabolism, HYDROGEN-PEROXIDE PRODUCTION, LIPID-PEROXIDATION, URINARY F-2-ISOPROSTANES, FLUORESCENT-PROBES, MASS-SPECTROMETRY, SUPEROXIDE ANION, REDOX REGULATION, QUANTIFICATION, OXIDANT, MARKER |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Genetics, Evolution and Environment UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10152301 |
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