Bhosale, Gauri;
Duchen, Michael R;
(2022)
Investigating Mitochondrial Ca²⁺ Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters.
Methods in Molecular Biology
, 2497
pp. 325-332.
10.1007/978-1-0716-2309-1_23.
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Abstract
Mitochondrial Ca2+ buffering is a hallmark of eukaryotic cellular physiology, contributing to the spatiotemporal shaping of the cytosolic Ca2+ signals and regulation of mitochondrial bioenergetics. Often, this process is altered in a pathological context; therefore, it can be scrutinized experimentally for therapeutic intervention. In this chapter, we describe fluorescence and bioluminescence measurement of mitochondrial Ca2+ in both isolated mitochondria and intact cells.
| Type: | Article |
|---|---|
| Title: | Investigating Mitochondrial Ca²⁺ Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters |
| Location: | United States |
| Open access status: | An open access version is available from UCL Discovery |
| DOI: | 10.1007/978-1-0716-2309-1_23 |
| Publisher version: | https://doi.org/10.1007/978-1-0716-2309-1_23 |
| Language: | English |
| Additional information: | This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. |
| Keywords: | Bioluminescence calcium sensing, Calcium-sensitive genetic probes, Fluorescence calcium imaging, Mitochondrial calcium, Calcium, Calcium Signaling, Cytosol, Fluorescent Dyes, Mitochondria |
| UCL classification: | UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences UCL UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences |
| URI: | https://discovery.ucl.ac.uk/id/eprint/10152155 |
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