UCL Discovery
UCL home » Library Services » Electronic resources » UCL Discovery

Assessing the Redox Status of Mitochondria Through the NADH/FAD²⁺ Ratio in Intact Cells

Chi, Haoyu; Bhosale, Gauri; Duchen, Michael R; (2022) Assessing the Redox Status of Mitochondria Through the NADH/FAD²⁺ Ratio in Intact Cells. Methods in Molecular Biology , 2497 pp. 313-318. 10.1007/978-1-0716-2309-1_21. Green open access

[thumbnail of Duchen_Assessing the redox status of mitochondria through the NADHFAD+ ratio in intact cells.pdf]
Preview
Text
Duchen_Assessing the redox status of mitochondria through the NADHFAD+ ratio in intact cells.pdf - Accepted Version

Download (259kB) | Preview

Abstract

This section aims to describe the measurement of NADH and FAD2+ levels in intact cells using fluorescence microscopy. Both NADH and FADH2 are major electron donors for the electron transport chain through shifting of their redox status. Furthermore, within their redox couples, only NADH and FAD2+ are fluorescent. Therefore, calibration of the NADH and FAD2+ fluorescence signal is a crucial factor in accurately assessing mitochondrial function and redox status.

Type: Article
Title: Assessing the Redox Status of Mitochondria Through the NADH/FAD²⁺ Ratio in Intact Cells
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-2309-1_21
Publisher version: https://doi.org/10.1007/978-1-0716-2309-1_21
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Autofluorescence, ETC, FAD2+, NADH, Redox status, Flavin-Adenine Dinucleotide, Microscopy, Fluorescence, Mitochondria, NAD, Oxidation-Reduction
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10152154
Downloads since deposit
61Downloads
Download activity - last month
Download activity - last 12 months
Downloads by country - last 12 months

Archive Staff Only

View Item View Item