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A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX

Chung, Chih-Yao; Duchen, Michael R; (2022) A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX. Methods in Molecular Biology , 2497 pp. 333-337. 10.1007/978-1-0716-2309-1_24. Green open access

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Abstract

Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.

Type: Article
Title: A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX
Location: United States
Open access status: An open access version is available from UCL Discovery
DOI: 10.1007/978-1-0716-2309-1_24
Publisher version: https://doi.org/10.1007/978-1-0716-2309-1_24
Language: English
Additional information: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
Keywords: Dihydroethidium, MitoSOX, Mitochondria, Plate reader, Reactive oxygen species, Ethidium, Oxidative Stress, Phenanthridines, Reactive Oxygen Species, Superoxides
UCL classification: UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences > Cell and Developmental Biology
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences
UCL
UCL > Provost and Vice Provost Offices > School of Life and Medical Sciences > Faculty of Life Sciences > Div of Biosciences
URI: https://discovery.ucl.ac.uk/id/eprint/10152151
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